To guarantee the performance of the test under development, scientists employed a system able to detect whether the three sequences used to recognize SARS-CoV-2 were present in other living organisms. With regard to the RT-PCR tests developed by the National Reference Center, the three sequences are not present simultaneously in any other organisms apart from SARS-CoV-2.
The test is then validated on primary samples (confirmed as positive and negative) to verify its specificity and sensitivity (no false positives or false negatives). Negative controls (here for example nose or throat samples taken before 2019) can help assess the risk of non-specific amplification.
Finally, it is advisable to use two different tests (the two tests developed by the CNR at the Institut Pasteur are named IP2 and IP4) on the same sample to guarantee the reliability of the result. This means that six sequences of the viral genome, rather than three, need to be recognized and amplified, thereby increasing the reliability of RT-PCR testing.
Pasteur institute isn't a company "lol man" https://en.wikipedia.org/wiki/Pasteur_Institute
The Pasteur Institute (French: Institut Pasteur) is a French non-profit private foundation dedicated to the study of biology, micro-organisms, diseases, and vaccines. It is named after Louis Pasteur, who invented pasteurization and vaccines for anthrax and rabies. The institute was founded on June 4, 1887, and inaugurated on November 14, 1888.
For over a century, the Institut Pasteur has researched infectious diseases. This worldwide biomedical research organization based in Paris was the first to isolate HIV, the virus that causes AIDS, in 1983. Over the years, it has been responsible for discoveries that have enabled medical science to control diseases as diphtheria, tetanus, tuberculosis, poliomyelitis, influenza, yellow fever, and plague.
Since 1908, ten Institut Pasteur scientists have been awarded the Nobel Prize for medicine and physiology—the 2008 Nobel Prize in Physiology or Medicine was shared between two Pasteur scientists.
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