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The product information pamphlet for AstraZeneca COVID-19 vaccine states

The vaccine is manufactured using material originally sourced from a human embryo (Human Embryonic Kidney cells: HEK293)

All the deboonking says vaccines don't contain any aborted fetal cells, "only" they are used to culture the virus, the most detailed answer I could find says the baby cells are "filtered out making it unlikely any is in the vaccine".

So my question is how is it cleaned/filtered?

My religious grandmother is seeking excuses to not take the vaccine, even though the church says it is okay.

The product information pamphlet for AstraZeneca COVID-19 vaccine states >The vaccine is manufactured using material originally sourced from a human embryo (Human Embryonic Kidney cells: HEK293) All the deboonking says vaccines don't contain any aborted fetal cells, "only" they are used to culture the virus, the most detailed answer I could find says the baby cells are "filtered out making it *unlikely* any is in the vaccine". So my question is *how* is it cleaned/filtered? My religious grandmother is seeking excuses to not take the vaccine, even though the church says it is okay.

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[–] • 0 pt

Thanks, that linked to https://www.nature.com/articles/s41586-020-2608-y which states

The virus was rescued and propagated in T-Rex HEK293 cells (Invitrogen) in which antigen expression during virus propagation is repressed. Purification was carried out using CsCl gradient ultracentrifugation. Virus titres were determined by hexon immunostaining assay and viral particles were calculated on the basis of spectrophotometry16,17.

I don't really understand that beyond the spun it really fast to separate different parts out.

[–] • 1 pt (edited )

They use these cell lines because they have so much experience in creating variants of these cells. The cell lines used in virus isolation and production are modified in a way that they don't fight the virus, so the virus replicates fast. Because they know these cell lines so well, they then can sort out the virus by eliminating everything else.