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125

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[–] [deleted] 2 pts

yes you would want to know the entire genomic sequence of a virus to target specific parts of the genome and avoid off target effects. when selecting primers and guide RNAs for CRISPR you need to target a unique sequence. the only way to ensure you're targeting a unique sequence is to look in the entire genome.

isolating and sequencing an entire viral genome is not difficult though. it's routine