It details a mechanism of delivering DNA into a cell. Cells have cell walls (a lipid bilayer) and if you want to put DNA inside the cell you have to get it through the cell wall without damaging it and without damaging the cell wall.
A cell can pull things inside of it through a process called endocytosis. Its an infold of the wall that pinches off inside the cell, and is the opposite of exocytosis. There is a image depiction of it
In this paper they are getting the cell to perform endocytosis on DNA that they want to put inside the cell. That DNA could have a variety of functions, basically whatever they want.
Normally once something is pulled into the cell through endocytosis, that thing is still surrounded by the bubble formed from the cell wall, as you can see in the illustration. That is called an endosome (edited to say that endosomes are very similar to vesicles, which is what it is called in that illustration). Normally the contents of endosomes/vesicles FIRST get digested and THEN studied by the cell so it knows its environment. If you left the DNA in that endosome it would be digested. However, they want it to get outside the endosome without digesting or damaging the DNA....so they are putting the superparamagnetic nanoparticles with the DNA, and once it is in the endosome in the cell, they are using magnetic waves to basically rip the iron particles through the endosome wall, effectively bursting it and releasing the DNA into the cytoplasm of the cell (like a mini shotgun blast ripping through drywall).
Once the DNA is in the cytoplasm unharmed, it has escaped digestion and it is then called a plasmid. A plasmid is DNA that is separate from chromosomal DNA, but can be processed like chromosomal DNA is. Its like piggyback DNA. It is usually found in bacteria.
Brilliant explanation thank you. So is there any danger to the vaccine doing this in your opinion?
i would think (with no direct experience) that you would need apply the magnetic field soon after the injection, otherwise i would think all the endosomes would get digested and their contents destroyed. But I have no direct experience with this sort of research. Since the current vaccine doesn't have a magnetic field step it probably isn't happening. For the current vaccine--at least mRNA usually eventually degrades, while DNA can hang around in a cell for a lot longer. That's not to say what the current vaccine does isn't concerning, because a lot of things can be affected long term without involving DNA
and i say usually eventually because thats normal cellular mRNA. But they know what kind of enzymes usually degrade mRNA so i have no doubt if you wanted to... you could protect it from those enzymes by adding or altering some of the domains that the enzymes recognize, and make the mRNA persist in the cytoplasm for longer than usual--if you wanted to.
hey i read today and it made me think of you, I thought you might like it.
Cool, thank you. Ive got it open in my browser and I’ll take a look tomorrow.
there are so many unexplored potential dangers it boggles the mind. there is nothing definitive you can point to which is why they say nothing has been proven.
So they’re putting alien eggs into our cells. Great.
i guess eggs in a plasmid sense yes
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